The Basic Principles Of 가슴수술
The Basic Principles Of 가슴수술
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Ideal results are acquired when restricting extended cell publicity to ambient temperature circumstances. Look at holding unused cells in a humidified incubator with five% CO2 at 37°C when doing larger sized experiments.
The in vitro differentiation of human pluripotent stem cells (hPSCs) into distinctive cell and tissue types enables the study of human biology without the need for primary tissues or in vivo designs.
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Set the “unstained” tube apart. With the “viability dye” sample, centrifuge the tube at three hundred x g
Consider the position by checking a droplet and stopping the dissociation when close to 80% in the cell suspension is solitary-celled. Keep away from about-digestion.
Differentiation into adipocytes, osteoblasts, or chondrocytes with suitable MesenCult™ differentiation systems
Yes, you’ll locate the techniques to complete an ICC staining with your epithelial cells cultured in the ALI in this protocol. Here's a list of antibodies which can be utilized for the characterization of airway cultures:
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Notes: Use pipette tips to gently scratch the bottom of every very well in order to lift the monolayer off, then dissociate the cell clumps into an individual-cell suspension by pipetting up and down five - ten periods.
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The subsequent fluorochrome-conjugated antibodies and dyes are advisable to facilitate Evaluation of gene-edited CD34+ HSPCs: